Applications: Difference between revisions

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Below you can find selected example applications, some of them have been published already.
Below you can find selected example applications, some of them have been published already.


== MiCA - MiToBo Cell Image Analyzer ==
== Neuron Analyzer 2D ==


presented at<br/>
The ''Neuron Analyzer 2D'' is available since release version 1.1 of MiToBo. [[File:NeuronAnalyzer2D.png|right|link=]]
''B. Möller and S. Posch,<br/>
'''"MiCA - Easy Cell Image Analysis with Normalized Snakes"'''.<br/>
Workshop on Microscopic Image Analysis with Applications in Biology (MIAAB '11), Heidelberg, Germany, September 2011.''


'''Name of Plugin:'''<br/>
'''Name of Plugin:'''<br/>
CellImageAnalyzer_2D (since MiToBo version 0.9.6)
NeuronAnalyzer_2D (since MiToBo version 1.1)


'''Main features:'''<br/>
'''Main features:'''<br/>
* Integrated analysis of multi-channel microscope images of cells
* Neuron boundary detection based on active contours
* Allows for segmentation of cells, nuclei and sub-cellular structures
* Identification of structural neuron parts, like soma, neurites and growth cones
* Techniques subsume active contours, wavelets, morphological operators, and others
* Morphology analysis, e.g., neurite length, average neurite width, number of branch and end points, growth cone size and shape roundness, etc.
* Visualization and quantitative summary of segmentation results
* Extraction of molecular profiles from fluorescently labeld molecules
* Detection of molecular particles, for example FISH data
 
'''Note:''' The application is currently only available on Linux OS.
 
'''Example images'''<br/>
...will be available soon


== Scratch assay analysis ==
== Scratch assay analysis ==
Line 36: Line 39:
[http://www.informatik.uni-halle.de/mitobo/downloads/scratch_examples.zip Scratch assay images]
[http://www.informatik.uni-halle.de/mitobo/downloads/scratch_examples.zip Scratch assay images]


== Neuron Analyzer 2D ==
== MiCA - MiToBo Cell Image Analyzer ==


The ''Neuron Analyzer 2D'' is available since release version 1.1 of MiToBo. [[File:NeuronAnalyzer2D.png|right|link=]]
presented at<br/>
''B. Möller and S. Posch,<br/>
'''"MiCA - Easy Cell Image Analysis with Normalized Snakes"'''.<br/>
Workshop on Microscopic Image Analysis with Applications in Biology (MIAAB '11), Heidelberg, Germany, September 2011.''


'''Name of Plugin:'''<br/>
'''Name of Plugin:'''<br/>
NeuronAnalyzer_2D (since MiToBo version 1.1)
CellImageAnalyzer_2D (since MiToBo version 0.9.6)


'''Main features:'''<br/>
'''Main features:'''<br/>
* Neuron boundary detection based on active contours
* Integrated analysis of multi-channel microscope images of cells
* Identification of structural neuron parts, like soma, neurites and growth cones
* Allows for segmentation of cells, nuclei and sub-cellular structures
* Morphology analysis, e.g., neurite length, average neurite width, number of branch and end points, growth cone size and shape roundness, etc.
* Techniques subsume active contours, wavelets, morphological operators, and others
* Extraction of molecular profiles from fluorescently labeld molecules
* Visualization and quantitative summary of segmentation results
* Detection of molecular particles, for example FISH data
 
'''Note:''' The application is currently only available on Linux OS.
 
'''Example images'''<br/>
...will be available soon

Revision as of 10:22, 21 February 2013

Several image processing pipelines have already been developed in MiToBo.
Below you can find selected example applications, some of them have been published already.

Neuron Analyzer 2D

The Neuron Analyzer 2D is available since release version 1.1 of MiToBo.

NeuronAnalyzer2D.png

Name of Plugin:
NeuronAnalyzer_2D (since MiToBo version 1.1)

Main features:

  • Neuron boundary detection based on active contours
  • Identification of structural neuron parts, like soma, neurites and growth cones
  • Morphology analysis, e.g., neurite length, average neurite width, number of branch and end points, growth cone size and shape roundness, etc.
  • Extraction of molecular profiles from fluorescently labeld molecules
  • Detection of molecular particles, for example FISH data

Note: The application is currently only available on Linux OS.

Example images
...will be available soon

Scratch assay analysis

published in
M. Glaß, B. Möller, A. Zirkel, K. Wächter, S. Hüttelmaier and S. Posch,
"Scratch Assay Analysis with Topology-preserving Level Sets and Texture Measures".
Proc. of Iberian Conference on Pattern Recognition and Image Analysis (IbPRIA '11), LNCS 6669, pp. 100-108, Springer, Las Palmas de Gran Canaria, Spain, June 2011.

Name of Plugin:
ScratchAssay_Analysis (since MiToBo version 0.9.5)

Description:

  • Quantifies the scratch area in monolayer cell culture images with levelset techniques
  • Combines the results from images of different time points in a results table


Example images
Scratch assay images

MiCA - MiToBo Cell Image Analyzer

presented at
B. Möller and S. Posch,
"MiCA - Easy Cell Image Analysis with Normalized Snakes".
Workshop on Microscopic Image Analysis with Applications in Biology (MIAAB '11), Heidelberg, Germany, September 2011.

Name of Plugin:
CellImageAnalyzer_2D (since MiToBo version 0.9.6)

Main features:

  • Integrated analysis of multi-channel microscope images of cells
  • Allows for segmentation of cells, nuclei and sub-cellular structures
  • Techniques subsume active contours, wavelets, morphological operators, and others
  • Visualization and quantitative summary of segmentation results