Applications/CellMigrationAnalyzer

Cell Migration Analyzer

Description:

• Segments and tracks fluorescently labeled cells from time lapse image sequences
• An additional fluorescence channel acting as a marker for including/ excluding certain cells can be used
• Descriptors characterizing migratory behaviour, morphological parameters and fluorescence distributions are reported in tab separated files that can be further processed by spreadsheet programs or R.

Name of Plugin/Operator:
de.unihalle.informatik.MiToBo.apps.singleCellTracking2D.CellMigrationAnalyzer
(since MiToBo version 1.3)

Additional operators contained in the package:

• FluorescentCellSegmenter
  • segments fluorescently labeled cells from images/ image sequences
• CellSegmentationPostprocessing
  • postprocesses objects from binary images/ image sequences (e.g. size filtering)
• CellTrackerBipartite
  • tracks (i.e. creates a label image sequence) objects from binary image sequences based on the objects' locations and areas
• MigrationAnalyzer
  • calculates migration and shape descriptors of objects from a label image sequence as well as intensity descriptors if a corresponding fluorescence image sequence is given
  • can create colored trajectories on a separate image or as overlay

Example images
tracking sequences