Applications

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Several image processing pipelines have already been developed in MiToBo.
Below you can find selected example applications, some of them have been published already.

MiCA - MiToBo Cell Image Analyzer

presented at
B. Möller and S. Posch,
"MiCA - Easy Cell Image Analysis with Normalized Snakes".
Workshop on Microscopic Image Analysis with Applications in Biology (MIAAB '11), Heidelberg, Germany, September 2011.

Name of Plugin:
CellImageAnalyzer_2D (since MiToBo version 0.9.6)

Main features:

  • Integrated analysis of multi-channel microscope images of cells
  • Allows for segmentation of cells, nuclei and sub-cellular structures
  • Techniques subsume active contours, wavelets, morphological operators, and others
  • Visualization and quantitative summary of segmentation results

Scratch assay analysis

published in
M. Glaß, B. Möller, A. Zirkel, K. Wächter, S. Hüttelmaier and S. Posch,
"Scratch Assay Analysis with Topology-preserving Level Sets and Texture Measures".
Proc. of Iberian Conference on Pattern Recognition and Image Analysis (IbPRIA '11), LNCS 6669, pp. 100-108, Springer, Las Palmas de Gran Canaria, Spain, June 2011.

Name of Plugin:
ScratchAssay_Analysis (since MiToBo version 0.9.5)

Description:

  • Quantifies the scratch area in monolayer cell culture images with levelset techniques
  • Combines the results from images of different time points in a results table


Example images
Scratch assay images