Applications/CellMigrationAnalyzer: Difference between revisions

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(Created page with "== Cell Migration Analysis == '''Name of Plugin/Operator:'''<br/> CellMigrationAnalyzer (since MiToBo version 1.3) '''Description:'''<br/> * Segments and tracks fluorescently ...")
 
 
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== Cell Migration Analysis ==
== Cell Migration Analyzer ==
[[File:tracking_ES-2.gif|256px|right|link=]]
[[File:speeds.png|256px|right|link=]]
[[File:directionalities.png|256px|right|link=]]


'''Description:'''<br/>
* Segments and tracks fluorescently labeled cells from time lapse image sequences
* An additional fluorescence channel acting as a marker for including/ excluding certain cells can be used
* Descriptors characterizing migratory behaviour, morphological parameters and fluorescence distributions are reported in tab separated files that can be further processed by spreadsheet programs or R.


'''Name of Plugin/Operator:'''<br/>
'''Name of Plugin/Operator:'''<br/>
CellMigrationAnalyzer (since MiToBo version 1.3)
<code>
de.unihalle.informatik.MiToBo.apps.singleCellTracking2D.CellMigrationAnalyzer
</code>
<br>
(since MiToBo version 1.3)
 
'''Additional operators contained in the package:'''<br/>
* FluorescentCellSegmenter
** segments fluorescently labeled cells from images/ image sequences
* CellSegmentationPostprocessing
** postprocesses objects from binary images/ image sequences (e.g. size filtering)
* CellTrackerBipartite
** tracks (i.e. creates a label image sequence) objects from binary image sequences based on the objects' locations and areas
* MigrationAnalyzer
** calculates migration and shape descriptors of objects from a label image sequence as well as intensity descriptors if a corresponding fluorescence image sequence is given
** can create colored trajectories on a separate image or as overlay


'''Description:'''<br/>
* Segments and tracks fluorescently labeled cells from time lapse image sequences
* Besides descriptors characterizing migratory behaviour, morphological as well as fluorescence changes are reported




'''Example images'''<br/>
'''Example images'''<br/>
[http://www.informatik.uni-halle.de/mitobo/downloads/tracking_examples.zip tracking images]
[http://www.informatik.uni-halle.de/mitobo/downloads/tracking_examples.zip tracking sequences]

Latest revision as of 08:40, 3 September 2014

Cell Migration Analyzer

Tracking ES-2.gif
Speeds.png
Directionalities.png


Description:

  • Segments and tracks fluorescently labeled cells from time lapse image sequences
  • An additional fluorescence channel acting as a marker for including/ excluding certain cells can be used
  • Descriptors characterizing migratory behaviour, morphological parameters and fluorescence distributions are reported in tab separated files that can be further processed by spreadsheet programs or R.

Name of Plugin/Operator:
de.unihalle.informatik.MiToBo.apps.singleCellTracking2D.CellMigrationAnalyzer
(since MiToBo version 1.3)

Additional operators contained in the package:

  • FluorescentCellSegmenter
    • segments fluorescently labeled cells from images/ image sequences
  • CellSegmentationPostprocessing
    • postprocesses objects from binary images/ image sequences (e.g. size filtering)
  • CellTrackerBipartite
    • tracks (i.e. creates a label image sequence) objects from binary image sequences based on the objects' locations and areas
  • MigrationAnalyzer
    • calculates migration and shape descriptors of objects from a label image sequence as well as intensity descriptors if a corresponding fluorescence image sequence is given
    • can create colored trajectories on a separate image or as overlay


Example images
tracking sequences