Applications/CellMigrationAnalyzer: Difference between revisions

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'''Description:'''<br/>
'''Description:'''<br/>
* Segments and tracks fluorescently labeled cells from time lapse image sequences
* Segments and tracks fluorescently labeled cells from time lapse image sequences
* Descriptors characterizing migratory behaviour, morphological parameters and fluorescence distributions are reported
* An additional fluorescence channel acting as a marker for including/ excluding certain cells can be used
* Descriptors characterizing migratory behaviour, morphological parameters and fluorescence distributions are reported in tab separated files that can be further processed by spreadsheet programs or R.


'''Name of Plugin/Operator:'''<br/>
'''Name of Plugin/Operator:'''<br/>
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<br>
<br>
(since MiToBo version 1.3)
(since MiToBo version 1.3)
'''Additional operators contained in the package:'''<br/>
* FluorescentCellSegmenter
** segments fluorescently labeled cells from images/ image sequences
* CellSegmentationPostprocessing
** postprocesses objects from binary images/ image sequences (e.g. size filtering)
* CellTrackerBipartite
** tracks (i.e. creates a label image sequence) objects from binary image sequences based on the objects' locations and areas
* MigrationAnalyzer
** calculates migration and shape descriptors of objects from a label image sequence as well as intensity descriptors if a corresponding fluorescence image sequence is given
** can create colored trajectories on a separate image or as overlay




'''Example images'''<br/>
'''Example images'''<br/>
[http://www.informatik.uni-halle.de/mitobo/downloads/tracking_examples.zip tracking images]
[http://www.informatik.uni-halle.de/mitobo/downloads/tracking_examples.zip tracking sequences]

Latest revision as of 08:40, 3 September 2014

Cell Migration Analyzer

Tracking ES-2.gif
Speeds.png
Directionalities.png


Description:

  • Segments and tracks fluorescently labeled cells from time lapse image sequences
  • An additional fluorescence channel acting as a marker for including/ excluding certain cells can be used
  • Descriptors characterizing migratory behaviour, morphological parameters and fluorescence distributions are reported in tab separated files that can be further processed by spreadsheet programs or R.

Name of Plugin/Operator:
de.unihalle.informatik.MiToBo.apps.singleCellTracking2D.CellMigrationAnalyzer
(since MiToBo version 1.3)

Additional operators contained in the package:

  • FluorescentCellSegmenter
    • segments fluorescently labeled cells from images/ image sequences
  • CellSegmentationPostprocessing
    • postprocesses objects from binary images/ image sequences (e.g. size filtering)
  • CellTrackerBipartite
    • tracks (i.e. creates a label image sequence) objects from binary image sequences based on the objects' locations and areas
  • MigrationAnalyzer
    • calculates migration and shape descriptors of objects from a label image sequence as well as intensity descriptors if a corresponding fluorescence image sequence is given
    • can create colored trajectories on a separate image or as overlay


Example images
tracking sequences