Applications/PaCeQuantToolset

The PaCeQuant Plugin and Corresponding Tools

The PaCeQuant plugin for fully automatic cell segmentation and shape feature extraction is part of MiToBo since release 1.8.6.
In MiToBo version 2.0 released in May 2020 it got an update which added the largest empty circle as shape criterion to PaCeQuant's feature set.

More details about the plugin and its usage can be found on the PaCeQuant's website.

Main publication:

• B. Möller, Y. Poeschl, R. Plötner, and K. Bürstenbinder,
  PaCeQuant: A Tool for High-Throughput Quantification of Pavement Cell Shape Characteristics.
  Plant Physiology, 175(1), September 2017.

Over the time the main plugin has been supplemented with several complementary tools to ease cell shape analysis and feature visualization:

• the FeatureColorMapper plugin for creating heatmap visualizations of cell features

• the LabelImageEditor for handy manual post-processing of segmentation results

• the R package PaCeQuantAna for statistical analysis of feature data and creation of publication-ready plots of feature distributions and statistical analysis results
  
  

Further references where PaCeQuant and the tools are documented or have been used:

• Y. Poeschl, B. Möller, L. Müller, and K. Bürstenbinder,
  User-friendly assessment of pavement cell shape features with PaCeQuant: Novel functions and tools.
  In Methods in Cell Biology, Academic Press, 2020.

• B. Möller, Y. Poeschl, S. Klemm, and K. Bürstenbinder,
  Morphological Analysis of Leaf Epidermis Pavement Cells with PaCeQuant.
  In F. Cvrčková and V. Žárský, editors, Plant Cell Morphogenesis: Methods and Protocols, chapter 22, pages 329-349, Springer New York, New York, NY, 2019.

• D. Mitra, S. Klemm, P. Kumari, J. Quegwer, B. Möller, Y. Poeschl, P. Pflug, G. Stamm, S. Abel, K. Bürstenbinder,
  Microtubule-associated protein IQ67 DOMAIN5 regulates morphogenesis of leaf pavement cells in Arabidopsis thaliana.
  In Journal of Experimental Botany, 70(2):529-543, January 2019.
  
  

PaCeQuant and the different tools have been developed in close collaboration with...

• the group of Dr. Katharina Bürstenbinder, Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany.
  

• Dr. Yvonne Pöschl-Grau, member of the Bioinformatics Unit (BIU) of the German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig